| 质粒名称: | R777-E011 Hs.ARHGAP35 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 70295 |
| 存储实验室: |
Dominic Esposito
|
|
载体骨架基本信息 |
|
| 载体名称: | pDonor-255 |
| 载体出品公司: | -- |
| 载体抗性: | 壮观霉素 |
| 空载体大小: | 2693 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Gateway Entry Clone |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ARHGAP35 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4500 bp |
| 基因突变: | -- |
| GenBank编号 | NM_004491.4 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG) |
| 3' 测序引物 | attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC) |
|
文献引用方法 |
|
| 材料和方法部分: | R777-E011 Hs.ARHGAP35 was a gift from Dominic Esposito (Addgene plasmid # 70295 ; http://n2t.net/addgene:70295 ; RRID:Addgene_70295) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS R777-E011 Hs.ARHGAP35 7193 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Gateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon
(for native or N-terminal fusions).
ACCESSION .
VERSION .
KEYWORDS R777-E011 Hs.ARHGAP35
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7193)
TITLE Esposito lab
REFERENCE 2 (bases 1 to 7193)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7193
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 23..45
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 37..54
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 38..54
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 84..183
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
RBS 4256..4264
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
protein_bind complement(4684..4783)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 5160..5951
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 6044..6632
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6533..6552
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6786..6803
/label=L4440
/note="L4440 vector, forward primer"
terminator 6962..6989
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 7081..7167
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
181 gcaccatgat gatggcaaga aagcaagatg tccgaattcc cacctacaac atcagtgtgg
241 tgggattatc tgggaccgag aaggaaaagg gccagtgtgg gattggaaag tcttgtttgt
301 gcaaccgctt cgtgcgcccg agtgctgacg agtttcactt ggaccatacc tccgtcctca
361 gcaccagtga ctttggaggg cgagtggtca ataatgacca ctttctctac tggggagaag
421 ttagccgctc cctggaggat tgtgtggaat gtaagatgca cattgtggag cagactgaat
481 ttattgatga tcagactttt caacctcatc gaagcacggc cctgcagccc tatatcaaga
541 gagctgctgc gaccaagctt gcatcagctg aaaaactcat gtacttttgc actgaccagc
601 tggggctgga gcaggacttt gagcagaaac aaatgccaga cggaaagctg ctggttgatg
661 gttttcttct tggtattgat gttagcaggg gcatgaatag gaactttgat gaccagctca
721 agtttgtctc caatctctac aatcagcttg caaaaacaaa aaagcccata gtggtggtcc
781 tgactaagtg tgacgaaggt gttgagcggt acattagaga tgcacatact tttgccttaa
841 gcaaaaagaa cctccaggtt gtggagacct cagcgagatc caatgtaaac gtggacttgg
901 ctttcagcac cttagtgcaa ctcattgata aaagtcgggg aaagacaaaa atcattcctt
961 attttgaagc tctcaagcag cagagtcagc agatagctac agcaaaagac aagtatgagt
1021 ggctggtgag tcgcattgtg aaaaaccaca atgagaactg gctgagtgtc agccgaaaga
1081 tgcaggcctc tccagaatac caggactatg tctacctgga agggactcag aaagccaaga
1141 agctgtttct acagcacatc caccgcctca agcatgagca tatcgagcgt aggagaaagc
1201 tgtacctggc agccctgcca ttagcttttg aagctcttat acctaatcta gatgaaatag
1261 accacctaag ctgcataaaa gccaaaaagc tcttagaaac caagccagaa ttcttgaagt
1321 ggtttgttgt gcttgaagag accccatggg atgccaccag tcacattgac aacatggaaa
1381 acgaacggat tccctttgat ttaatggata ccgtccctgc agagcagcta tacgaggccc
1441 acttagagaa gctgaggaac gaaaggaaaa gagttgagat gcgaagggcg tttaaagaaa
1501 acctggagac ttctcctttc ataactcccg gaaagccttg ggaagaggcc cgtagtttta
1561 ttatgaatga ggatttctac cagtggctgg aggaatctgt atacatggat atttatggca
1621 aacaccaaaa gcaaattata gataaagcaa aggaagaatt tcaggagttg cttttggaat
1681 attcagaatt gttttatgaa ctggagctgg atgctaagcc cagcaaggag aagatgggtg
1741 ttattcagga tgttctggga gaggaacagc gatttaaagc attacaaaag ctccaagcag
1801 agcgtgatgc ccttattctg aaacacattc attttgtgta ccacccaaca aaggagacat
1861 gccccagctg cccagcttgt gtggacgcta agattgagca cttgattagt tctcggttta
1921 tccggccgtc tgaccggaat cagaaaaatt cactctctga ccctaacatt gatagaatca
1981 acttggttat attgggcaaa gacggccttg cccgagagtt ggccaatgag attcgagctc
2041 tttgtacaaa tgatgacaag tatgtgatag atggtaaaat gtatgagctt tccctgaggc
2101 caatagaggg gaatgtcagg cttcctgtga actctttcca gacgccaaca tttcagcccc
2161 acggctgtct ctgcctttac aattcaaagg aatcgctatc ctatgtagtg gaaagtatag
2221 agaagagtag agagtccacg ctgggccggc gggataatca tttagtccat ctccccctta
2281 cattaatttt ggttaacaag agaggagaca ccagtggaga gactctgcat agcttaatac
2341 agcaaggtca acaaattgct agcaaacttc agtgtgtctt tctcgaccct gcttctgctg
2401 gcattggtta cggacgcaac attaatgaaa agcaaatcag tcaagttttg aagggactcc
2461 tggactctaa gcgtaactta aacctggtca gttctactgc tagcatcaaa gatttggctg
2521 atgttgatct gcgaattgtt atgtgtctga tgtgtggaga tccttttagt gcagatgaca
2581 tactttttcc tgtccttcag tcccaaacct gtaaatcttc ccattgtgga agcaacaact
2641 ctgttttact tgaactacca atcggactgc acaagaagcg gattgaactg tctgttcttt
2701 cataccattc ctcctttagc atcagaaaga gccggttggt tcatgggtac attgtttttt
2761 attcagccaa acgtaaggcc tctttggcta tgttacgtgc ctttctttgt gaagtgcagg
2821 atattatccc tattcagctt gtagcactca ctgatggcgc tgtagatgtc ctggacaatg
2881 acttaagtag ggaacagcta actgaggggg aggagattgc tcaagaaatt gacggaaggt
2941 tcacaagcat cccctgtagc caaccccagc ataaacttga gatctttcac ccatttttta
3001 aagatgtggt ggaaaaaaag aacataatcg aggctactca tatgtacgat aatgctgccg
3061 aggcctgtag caccaccgaa gaggtgttta actccccccg ggcaggatca ccgctctgca
3121 actcaaacct gcaggattca gaagaagata tcgagccatc ttacagcctg tttcgagaag
3181 acacatcact gccttctctg tccaaagacc attctaagct ctctatggaa ctggagggaa
3241 atgatgggct gtctttcatt atgagcaatt ttgagagtaa actgaacaac aaagtacctc
3301 cgccagtcaa accaaagcct cctgtccatt ttgaaattac aaagggggat ctatcttatt
3361 tagaccaagg ccatagggat ggacagagga agtctgtgtc ttctagcccc tggctgcctc
3421 aggatgggtt tgatccttct gactatgctg aacccatgga tgctgtggtg aagccaagga
3481 atgaagaaga aaacatatac tccgtgcccc atgacagcac ccaaggcaaa atcatcacca
3541 ttcggaatat caacaaagcc cagtccaacg gcagcgggaa tggttctgac agtgaaatgg
3601 acaccagctc tctagagcga gggcgcaagg tttccatcgt gagcaagcca gtgctgtaca
3661 ggacgagatg cacccggctg gggcggtttg ctagttaccg gaccagcttc agcgtgggga
3721 gtgatgatga gctggggccc atccggaaga aagaggagga tcaggcatcc cagggttata
3781 aaggggacaa tgctgtcatt ccatacgaaa cagacgaaga cccgcggagg aggaatattc
3841 ttcgcagcct aaggaggaac actaagaaac caaagcccaa accccggcca tccatcacaa
3901 aggcaacctg ggagagtaac tattttgggg tgcccttaac aactgtcgtg actccagaga
3961 agccgatccc catttttatt gaaagatgta ttgagtacat tgaagccaca ggactgagca
4021 cggaaggcat ctaccgggtc agcgggaaca agtctgagat ggagagtctg cagagacagt
4081 ttgatcaaga ccacaacctg gacctggcag agaaagactt tacggtgaat accgtggctg
4141 gtgccatgaa gagctttttc tcagaactgc ctgaccccct ggtcccgtat aacatgcaga
4201 tcgacttggt ggaagcacac aaaatcaacg accgggagca gaagttgcat gcccttaagg
4261 aggtattaaa gaaatttcca aaggaaaacc acgaagtctt caagtatgtc atctctcacc
4321 taaacaaggt cagccacaac aacaaggtga atctcatgac cagcgagaac ctctccatct
4381 gcttctggcc caccttgatg agacctgatt tcagcactat ggacgccctc acagccacgc
4441 gcacctacca gacaatcatt gaactcttta tccagcagtg ccccttcttc ttctacaatc
4501 ggcccatcac cgagcccccc ggcgccaggc ccagctcccc ctctgccgtg gcttccaccg
4561 tccccttcct cacttccacg cctgtcacaa gtcagccgtc gcccccacag tcgcctccac
4621 ccacccccca gtccccaatg cagccactgc ttccctccca gcttcaagcc gaacacacgc
4681 tgtaatcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
4741 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
4801 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
4861 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
4921 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
4981 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
5041 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
5101 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
5161 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
5221 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
5281 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
5341 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
5401 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
5461 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
5521 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
5581 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
5641 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
5701 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
5761 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
5821 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
5881 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
5941 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
6001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6181 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6421 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
6481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
6541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
6601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
6661 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
6721 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
6781 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
6841 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
6901 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
6961 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
7021 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
7081 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
7141 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
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